Ferrell, in an attempt to promote his latest film, was joined by some 1,580 people in superhero costumes at the Staples Center in Los Angeles on October 2.
The previously-held record had 1,500 people dressed in costumes.
The event was staged by Ferrell and DreamWorks Animation to promote the latest villain comedy MegaMind, in which Ferrell lent his voice to the main character for which the movie is named.
“Everyone in a Spider-Man outfit wins a new car!” Ferrell announced, jokingly, before quickly checking off-stage. “No? … No, OK. I was mistaken,” Farrell said, accompanied by blaring dance music, cheerleaders, and confetti-shooting cannons.
USA Today noted that there were numerous Superman and Batman costumes worn among people in the crowd.
“It was pretty much a haze, a sea of faces. It was tough to concentrate,” Farrell told USA Today. “I think I saw a (good) Captain America out there, and the Chef from South Park. Is that really a superhero? I don’t know. But that was a good costume.”